SDCBP antibody (N-Term)

Catalog No. ABIN185728

This Goat Polyclonal antibody specifically detects SDCBP in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SDCBP antibody (N-Term) (ABIN185728)

Target: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SDCBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SDCBP Antibody

Binding Specificity: N-Term

Purpose: Syntenin / SDCBP

Sequence: SLEDLKVDKV IQAQ

Specificity: This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SLEDLKVDKVIQAQ-C, from the N Terminus of the protein sequence according to NP_005616.2, NP_001007068.1, NP_001007069, NP_001007070.1, NP_001007071.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 10 μg/mL.

Western Blot: Approx 28-30 kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 32.4 kDa according to NP_005616.2 and NP_001007068.1 and of 32.3 kDa according to NP_001007070.1 and NP_001007071.1). Recommended concentration: 0.01-0.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SDCBP

Target: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Alternative Name: SDCBP

Background: SDCBP, Syntenin, syndecan binding protein (syntenin), MDA-9, ST1, SYCL, TACIP18 , melanoma differentiation associated protein-9, pro-TGF-alpha cytoplasmic domain-interacting protein 18, scaffold protein Pbp1 syntenin

Gene ID: 6386, 53378, 83841

NCBI Accession: NP_005616, NP_001007068, NP_001007069, NP_001007070, NP_001007071